Defective Interfering Particles of Encephalomyocarditis Virus

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Population

HeLa, L929 and BHK-21 cells infected with Encephalomyocarditis virus and five temperature-sensitive mutants

Comparison

Serial passage numerous times vs Standard EMC virus replication

Design

Preclinical

Authors

Discussion

May guide attenuated EMC virus strain development in vitro; leaves open relevance to in vivo pathogenesis or clinical translation.

Population

HeLa, L929 and BHK-21 cells infected with Encephalomyocarditis (EMC) virus and five temperature-sensitive mutants

Intervention

Serial passage numerous times

Comparator

Standard EMC virus replication

Outcome

Generation of defective interfering particles (presence of lower molecular weight RNA and interference with standard virion replication)surrogate

Serial passage of EMC virus in cell lines generates defective interfering particles with lower molecular weight RNA.

Radloff et al. (1983) studied this question.

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