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December 1, 2022Annals of Translational MedicineOpen Access

Electroacupuncture alleviates neuropathic pain caused by spared nerve injury by promoting AMPK/mTOR-mediated autophagy in dorsal root ganglion macrophage

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Authors

QXQian XuCNCong NiuJLJiajing Li

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Overview

Animal experiment demonstrates that electroacupuncture alleviates nerve injury pain in rats, indicating an analgesic role for macrophage autophagy.

Key Points

  • To examine whether electroacupuncture relieves spared nerve injury-induced neuropathic pain by regulating autophagy in dorsal root ganglion macrophages via the AMPK/mTOR pathway.
  • Induced neuropathic pain in rats via spared nerve injury (SNI) and administered electroacupuncture at the Zusanli (ST36) and Huantiao (GB30) acupoints seven days post-surgery.
  • Assessed mechanical hyperalgesia and tracked autophagic markers (p62, Beclin-1, and LC3-II/LC3-I) in L4-L6 dorsal root ganglia using immunofluorescence and Western blotting.
  • Investigated AMPK/mTOR pathway involvement using transmission electron microscopy and the AMPK inhibitor compound C.
  • Electroacupuncture significantly reduced mechanical hyperalgesia compared to untreated nerve-injured rats.
  • Autophagy substrate p62 selectively colocalized with the macrophage marker CD11b in dorsal root ganglia rather than neurons or satellite glial cells.
  • Electroacupuncture reversed nerve injury-induced increases in p62 while upregulating Beclin-1 and LC3-II/LC3-I ratios, an effect mediated by AMPK activation.

Cite This Study

Xu et al. (2022) studied this question.

synapsesocial.com/papers/6a8afa1a2ff0ab4f91e94b8ahttps://doi.org/10.21037/atm-22-5920
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Also Consider

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