February 1, 1993Molecular and Cellular Biology

Different regulatory sequences control creatine kinase-M gene expression in directly injected skeletal and cardiac muscle.

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Population

Adult rat cardiac and skeletal muscle

Comparison

Direct injection of plasmid DNA constructs… vs Different deletion constructs and comparison…

Design

Preclinical

Authors

CVC K VincentUniversity School AGAntonio GualbertoUniversity of North Carolina at Chapel Hill CPC V PatelUniversity School

Discussion

Member takes

Implication

Distinct cardiac-skeletal regulatory programs for creatine kinase-M warrant caution against clinical extrapolation; leaves open cardiac-selective enhancer targeting in humans.

Structured PICO

Population

Adult rat cardiac and skeletal muscle

Intervention

Direct injection of plasmid DNA constructs containing rabbit MCK gene promoter and enhancer sequences (and deletion variants)

Comparator

Different deletion constructs and comparison between skeletal and cardiac muscle tissues

Outcome

Expression of a downstream reporter genesurrogate

The creatine kinase-M gene is controlled by distinct regulatory programs in adult cardiac versus skeletal muscle, with a specific CArG sequence motif being essential for cardiac expression.

Cite This Study

Vincent et al. (1993) studied this question.

synapsesocial.com/papers/6a8b0ab7c12eaf65c8b1f674 https://doi.org/10.1128/mcb.13.2.1264

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Implication

Distinct cardiac-skeletal regulatory programs for creatine kinase-M warrant caution against clinical extrapolation; leaves open cardiac-selective enhancer targeting in humans.

Different regulatory sequences control creatine kinase-M gene expression in directly injected skeletal and cardiac muscle.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Different regulatory sequences control creatine kinase-M gene expression in directly injected skeletal and cardiac muscle.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider