Key result
The liquid-phase blocking ELISA showed less variation and higher discriminatory capacity for foot-and-mouth disease virus serotype A vaccine matching compared to neutralization tests.
Population
Cattle vaccinated with binary ethylenimine inactivated vaccines prepared from 10 different foot-and-mouth…
Comparison
Liquid-phase blocking enzyme-linked… vs Virus neutralization test and neutralization…
Design
Preclinical
Follow-up
3 weeks postvaccination
Authors
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May support refined FMDV serotype A vaccine matching; leaves open field validation before replacing neutralization tests.
The liquid-phase blocking ELISA is superior to neutralization tests for determining antigenic matching of FMD virus serotype A vaccines due to lower variability and higher discriminatory capacity.
Tekleghiorghis et al. (2014) studied Foot-and-mouth disease (FMD) virus serotype A vaccine matching (n=50). Liquid-phase blocking ELISA vs. Virus neutralization test and neutralization index test was evaluated on Relationship coefficient (r1) values and discriminatory capacity via ROC analysis. The liquid-phase blocking ELISA showed less variation and higher discriminatory capacity for foot-and-mouth disease virus serotype A vaccine matching compared to neutralization tests.