Key result
An electrochemical assay using standard glucose meters detected 19 influenza strains in one hour with a limit of detection of 10^2 plaque forming units.
Current user-friendly glucose meters can be repurposed to rapidly detect influenza viruses and measure antiviral drug susceptibility.
May enable rapid point-of-care influenza detection; hypothesis-generating from animal data and leaves open human validation.
An electrochemical assay has been designed to rapidly diagnose influenza viruses. Exposure of a glucose‐bearing substrate to influenza viruses or its enzyme, neuraminidase (NA), releases glucose, which was detected amperometrically. Two methods were used to detect released glucose. First, we used a standard glucose blood meter to detect two viral NAs and three influenza strains. We also demonstrated drug susceptibility of two antivirals, Zanamivir and Oseltamivir, using the assay. Finally, we used disposable test strips to detect nineteen H1N1 and H3N2 influenza strains using this assay in one hour. The limit and range of detection of this first generation assay is 102 and 102–108 plaque forming units (pfu), respectively. Current user‐friendly glucose meters can be repurposed to detect influenza viruses.
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Zhang et al. (2015) studied Influenza. Electrochemical assay using a glucose-bearing substrate was evaluated on Limit and range of detection of influenza viruses. An electrochemical assay using standard glucose meters detected 19 influenza strains in one hour with a limit of detection of 10^2 plaque forming units.
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