Key result
An electrochemical assay using a glucose-bearing substrate successfully detected 19 H1N1 and H3N2 influenza strains in one hour with a limit of detection of 10(2) plaque forming units.
Why the study?
Can an electrochemical assay using a glucose meter detect influenza viruses and measure drug susceptibility?
Can an electrochemical assay using a glucose meter detect influenza viruses and measure drug susceptibility?
Current user-friendly glucose meters can be repurposed to rapidly detect influenza viruses and measure antiviral drug susceptibility.
May enable glucose meter repurposing for influenza detection; leaves open clinical validation from animal data.
An electrochemical assay has been designed to rapidly diagnose influenza viruses. Exposure of a glucose-bearing substrate to influenza viruses or its enzyme, neuraminidase (NA), releases glucose, which was detected amperometrically. Two methods were used to detect released glucose. First, we used a standard glucose blood meter to detect two viral NAs and three influenza strains. We also demonstrated drug susceptibility of two antivirals, Zanamivir and Oseltamivir, using the assay. Finally, we used disposable test strips to detect nineteen H1N1 and H3N2 influenza strains using this assay in one hour. The limit and range of detection of this first generation assay is 10(2) and 10(2)-10(8) plaque forming units (pfu), respectively. Current user-friendly glucose meters can be repurposed to detect influenza viruses.
No takes yet. Share an insight, caveat, or question.
Zhang et al. (2015) studied Influenza. Electrochemical assay using a glucose-bearing substrate was evaluated on Detection of influenza viruses and measurement of drug susceptibility. An electrochemical assay using a glucose-bearing substrate successfully detected 19 H1N1 and H3N2 influenza strains in one hour with a limit of detection of 10(2) plaque forming units.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: