Key result
A universal immunosensor using electrochemical impedance spectroscopy detected all influenza A serotypes with sensitivity similar to molecular methods in under an hour.
Why the study?
Does a universal immunosensor using EIS and anti-M1 antibodies allow rapid and sensitive detection of influenza A virus?
Does a universal immunosensor using EIS and anti-M1 antibodies allow rapid and sensitive detection of influenza A virus?
A novel universal immunosensor using EIS and anti-M1 antibodies enables rapid, sensitive detection of all influenza A serotypes, facilitating point-of-care diagnostics.
May support rapid point-of-care influenza testing; animal data leaves open human validation before practice change.
Influenza is a contagious disease caught by humans caused by viruses belonging to the family Orthomyxoviridae. Each year, the influenza virus infects millions of people and kills hundreds of thousands of them. Traditional diagnostic methods, such as virus propagation and isolation, antigen capture immunoassays and molecular methods are not sufficient for the detection of the influenza virus. Development of a valid diagnostic assay for quick detection (in less than an hour) of the virus, with high sensitivity, is a challenge for researchers all over the world. Here we present a new, universal immunosensor for detection of the influenza A virus. By using electrochemical impedance spectroscopy (EIS) and direct attachment of antibodies to the gold electrode the assay allows detection of the pathogen with sensitivity similar to molecular methods in relatively short time. Application of universal anti-M1 antibodies allows detection of all serotypes of influenza A virus. The simple design of the sensor facilitates miniaturization of the device and its implementation for routine diagnostics during first contact with the patient, before applying a proper treatment.
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Nidzworski et al. (2014) studied Influenza A virus. Universal immunosensor using electrochemical impedance spectroscopy and anti-M1 antibodies vs. Traditional diagnostic methods (molecular methods) was evaluated on Detection of influenza A virus. A universal immunosensor using electrochemical impedance spectroscopy detected all influenza A serotypes with sensitivity similar to molecular methods in under an hour.
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