What question did this study set out to answer?

To determine the effects of the dihydropyridine calcium channel agonist Bay K 8644 on calcium uptake and prolactin secretion in normal and transformed rat pituitary cells.

January 1, 1986AJP Cell Physiology

Interaction of dihydropyridine Ca2+ agonist Bay K 8644 with normal and transformed pituitary cells

View Full Paper

Ask AI

Bookmark

Authors

JEJohn J. EnyeartThe Ohio State University Wexner Medical Center TAToru AizawaMukogawa Women's University PHPatricia M. HinkleUniversity of Rochester Medical Center

Discussion

Member takes

Implication

In vitro study demonstrates enhanced calcium uptake and prolactin secretion by Bay K 8644 in rat pituitary cells, indicating voltage-sensitive calcium channel modulation across endocrine tissues.

Key Points

To determine the effects of the dihydropyridine calcium channel agonist Bay K 8644 on calcium uptake and prolactin secretion in normal and transformed rat pituitary cells.
Treated GH4C1 rat pituitary tumor cells with Bay K 8644 concentrations ranging from 10⁻⁹ to 10⁻⁵ M, alone or combined with depolarizing KCl and the antagonist nisoldipine, to measure 45Ca2+ uptake and prolactin secretion.
Assessed prolactin release from normal rat pituitary tissue maintained in static cell culture and in dynamic perifusion systems.
Bay K 8644 produced a dose-dependent increase in 45Ca2+ uptake between 10⁻⁹ and 10⁻⁵ M in GH4C1 cells that strongly correlated with prolactin release.
Combining Bay K 8644 with depolarizing concentrations of KCl yielded supra-additive effects on calcium uptake and hormone secretion, which were competitively blocked by nisoldipine (depolarization IC50: 2 nM).
Bay K 8644 stimulated prolactin secretion in both cell culture and perifusion systems containing normal rat pituitary cells.

Cite This Study

Enyeart et al. (1986) studied this question.

synapsesocial.com/papers/6a8b28c4b676b7d1a2fb592d https://doi.org/10.1152/ajpcell.1986.250.1.c95

View Full Paper

Ask AI

Bookmark

Implication

In vitro study demonstrates enhanced calcium uptake and prolactin secretion by Bay K 8644 in rat pituitary cells, indicating voltage-sensitive calcium channel modulation across endocrine tissues.

Interaction of dihydropyridine Ca2+ agonist Bay K 8644 with normal and transformed pituitary cells

Discussion

Member takes

Implication

Key Points

Cite This Study

Interaction of dihydropyridine Ca2+ agonist Bay K 8644 with normal and transformed pituitary cells

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider