What question did this study set out to answer?

To determine how oxygen free radicals and ischemia-reperfusion impair renal membrane-bound Na+-K+-ATPase activity and alter membrane lipid integrity.

February 1, 1988AJP Cell Physiology

Depression of membrane-bound Na+-K+-ATPase activity induced by free radicals and by ischemia of kidney

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Authors

KKK. J. KakoUniversity of Zurich MKM. KatoTokai Gakuin University TMT. MatsuokaKagoshima University

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Implication

Experimental study reveals that free radicals and ischemia depress renal Na+-K+-ATPase activity via sulfhydryl loss and lipid peroxidation, indicating oxidative stress impairs ion transport.

Key Points

To determine how oxygen free radicals and ischemia-reperfusion impair renal membrane-bound Na+-K+-ATPase activity and alter membrane lipid integrity.
Incubated membrane-bound Na+-K+-ATPase from porcine kidney outer medulla with iron chloride, ADP, and hydrogen peroxide (0.1–100 mM) or exposed it to 254 nm ultraviolet light, with or without antioxidants (dithiothreitol or butylated hydroxytoluene).
Analyzed microsomal fractions prepared from canine kidney outer medulla following 1 hour of unilateral ischemia and 1 hour of reperfusion.
Hydrogen peroxide and ultraviolet exposure decreased ouabain-sensitive Na+-K+-ATPase activity, turnover rates, and sulfhydryl content in a concentration- and time-dependent manner while generating malondialdehyde and conjugated dienes.
Treatment with 10 mM dithiothreitol restored enzyme activity, turnover rate, and sulfhydryl levels without lowering malondialdehyde, whereas 2 mM butylated hydroxytoluene inhibited lipid peroxidation and fully restored ATPase activity in ultraviolet-treated membranes.
Ischemia and reperfusion in canine kidneys caused comparable membrane injury, significantly depressing Na+-K+-ATPase activity and sulfhydryl groups while promoting malondialdehyde generation.

Cite This Study

Kako et al. (1988) studied this question.

synapsesocial.com/papers/6a8b93229d5fb440a3beadd4 https://doi.org/10.1152/ajpcell.1988.254.2.c330

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Implication

Experimental study reveals that free radicals and ischemia depress renal Na+-K+-ATPase activity via sulfhydryl loss and lipid peroxidation, indicating oxidative stress impairs ion transport.

Depression of membrane-bound Na+-K+-ATPase activity induced by free radicals and by ischemia of kidney

Discussion

Member takes

Implication

Key Points

Cite This Study

Depression of membrane-bound Na+-K+-ATPase activity induced by free radicals and by ischemia of kidney

Discussion

Member takes

Implication

Key Points

Cite This Study

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