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May 1, 2020Thrombosis and HaemostasisOpen Access

Platelet Activation via Shear Stress Exposure Induces a Differing Pattern of Biomarkers of Activation versus Biochemical Agonists

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Why the study?

Underlying mechanisms of shear-mediated platelet activation and useful biomarkers to detect and discriminate mechanical versus biochemical platelet activation are poorly defined.

Does mechanical shear stress induce a differing pattern of biomarkers of activation compared to biochemical agonists in human platelets?

Population

Gel-filtered human platelets

Comparison

Mechanical shear stress activation vs biochemical agonists

Design

Preclinical in vitro experimental study

Authors

YRYana Roka‐MoiiaUniversity of ArizonaRWRyan M. WalkUniformed Services University of the Health SciencesDPDaniel E. PalomaresUniversity of Arizona

Discussion

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Member takes

Overview

Distinct shear-induced platelet phenotype may aid device thrombosis detection; hypothesis-generating in animal models, requiring human validation.

Structured PICO

Does mechanical shear stress induce a differing pattern of biomarkers of activation compared to biochemical agonists in human platelets?

P
Population
Gel-filtered human platelets
I
Intervention
Mechanical activation via either uniform constant or dynamic shear stress (including circulation through a ventricular assist device-propelled circulatory loop)
C
Comparator
Biochemical activation by adenosine diphosphate (ADP), thrombin receptor-activating peptide 6 (TRAP-6), thrombin, collagen, epinephrine, or arachidonic acid
O
Outcome
Pattern of biomarkers of platelet activation (P-selectin, integrin αIIbβ3 activation), apoptosis (mitochondrial membrane potential, caspase 3 activation, phosphatidylserine externalization), and procoagulant activitysurrogate

Shear-mediated platelet activation produces a distinct biomarker phenotype (high phosphatidylserine externalization and thrombin generation, low αIIbβ3 and P-selectin) compared to biochemical activation, which may help detect device-associated thrombosis.

Cite This Study

Roka‐Moiia et al. (2020) studied this question.

synapsesocial.com/papers/6a8ba57ceada60f85de3c2a8https://doi.org/10.1055/s-0040-1709524
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Arachidonic acid-induced calcium influx in human platelets. Comparison with the effect of thrombin1990 · 46 citations
  2. 2Quantification of Shear‐Induced Platelet Activation: High Shear Stresses for Short Exposure Time2015 · 88 citations
  3. 3Platelet receptors and signaling in the dynamics of thrombus formation2009 · 417 citations
  4. 4Annexin V as a probe of aminophospholipid exposure and platelet membrane vesiculation: a flow cytometry study showing a role for free sulfhydryl groups1993 · 357 citations
  5. 5Platelet Secretion Defects and Acquired von Willebrand Syndrome in Patients With Ventricular Assist Devices2018 · 59 citations