Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
February 15, 1979International Journal of Cancer

Enhanced induction of growth of B lymphoblasts from fresh human blood by primate type‐C retroviruses (gibbon ape leukemia virus and simian sarcoma virus)

View Full Paper
Ask AI
Bookmark
Share

Authors

PMP. D. MarkhamNational Institutes of HealthFRFrancis W. RuscettiUniversity of Maryland, BaltimoreSSS. Zaki SalahuddinSacred Heart College

Discussion

Loading...

Member takes

Overview

In vitro study shows primate type-C retroviruses induce B-lymphoblast growth in EBV-seropositive human blood, suggesting retroviruses can facilitate human lymphocyte transformation.

Key Points

  • To determine whether primate type-C retroviruses enhance the growth induction and transformation of human B lymphoblasts from fresh peripheral blood.
  • Exposed fresh peripheral blood leukocytes from EBV-seropositive donors (N=21), leukemic patients, and EBV-seronegative donors (N=8) to GALV, SiSV/SiSAV, BaEV, FeLV, or uninfected control cultures.
  • Characterized established cell lines using morphological, cytochemical, immunological (EBNA, EAC-rosette, E-rosette, surface immunoglobulins), and Giemsa-banded karyotypic analyses.
  • Assessed in vivo tumorigenicity by inoculating cells into 1- to 3-week-old athymic nude mice and measuring colony formation in semi-solid medium.
  • Cell lines were established in 52.4% (11 of 21) of EBV-seropositive donors exposed to GALV-SiSV/SiSAV, compared to less than 10% with BaEV, FeLV, or uninfected controls, and 0% (0 of 8) in EBV-seronegative donors.
  • Established lines were identified as polyclonal, karyotypically normal diploid B lymphoblasts expressing EBV nuclear antigen and surface immunoglobulins.
  • Approximately 80% of tested cultures formed colonies in semi-solid medium and produced locally invasive tumors in athymic nude mice while retaining their lymphoblastic properties and normal karyotype.

Cite This Study

Markham et al. (1979) studied this question.

synapsesocial.com/papers/6a8bbd9990fae8fd47e98e36https://doi.org/10.1002/ijc.2910230203
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Erythroid colony induction without erythropoietin by Friend leukemia virus in vitro.1975 · 40 citations
  2. 2One-Way Stimulation in Mixed Leukocyte Cultures1966 · 528 citations
  3. 3RECENT PROGRESS IN EPSTEIN-BARR VIRUS RESEARCH1977 · 103 citations
  4. 4Functional and Morphologic Characterization of Human T Cells Continuously Grown in Vitro1977 · 408 citations
  5. 5Contrasting Characteristics of Marek’s Disease Herpesvirus Isolated from Chickens with and without Avian Leukosis Virus Infection1978 · 4 citations