What question did this study set out to answer?

To examine whether endogenous nitric oxide modulates adrenergic nerve-mediated vasoconstriction in isolated guinea-pig pulmonary arteries.

October 1, 1991British Journal of PharmacologyOpen Access

Endogenous nitric oxide modulates adrenergic neural vasoconstriction in guinea‐pig pulmonary artery

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Population

Guinea-pig isolated pulmonary artery

Comparison

L-NMMA, exogenous noradrenaline, phenylephrine… vs Control conditions

Design

Preclinical

Authors

SLS.F. LiuLung Institute DCDavid CrawleyForeign and Commonwealth Office TETW EvansRoyal Brompton Hospital

Discussion

Member takes

Implication

Animal data support NO modulation of pulmonary adrenergic tone; hypothesis-generating and leaves open human translation.

Key Points

To examine whether endogenous nitric oxide modulates adrenergic nerve-mediated vasoconstriction in isolated guinea-pig pulmonary arteries.
Measured contractile responses of isolated guinea-pig pulmonary artery rings to electrical field stimulation (EFS) and exogenous vasoconstrictors, including noradrenaline, phenylephrine, and prostaglandin F2α.
Tested the effects of nitric oxide synthase inhibition using L-NMMA (0.3–100 µM), enantiomeric reversal with L-arginine, cyclo-oxygenase inhibition with indomethacin, and endothelial denudation.
L-NMMA concentration-dependently amplified EFS-induced contractions with a 3.4 ± 0.5-fold increase at 100 µM (n=6), which was completely reversed by L-arginine but not D-arginine.
L-NMMA (30 µM) significantly augmented contractions to exogenous noradrenaline (2.9 ± 0.6-fold increase, n=5), phenylephrine, and prostaglandin F2α to an equivalent extent.
Indomethacin had no significant effect on contractions, while endothelial removal abolished α2-adrenoceptor-mediated vasorelaxation, confirming a postjunctional nitric oxide mechanism without prostacyclin involvement.

Structured PICO

Population

Guinea-pig isolated pulmonary artery

Intervention

L-NMMA (0.3-100 microM), exogenous noradrenaline, phenylephrine, prostaglandin F2 alpha, UK14304, and indomethacin

Comparator

Control conditions (absence of inhibitors/modulators)

Outcome

Contractile response to electrical field stimulation (EFS) or exogenous agonistssurrogate

Endogenous nitric oxide inhibits the contractile response to adrenergic nerve stimulation in the guinea-pig pulmonary artery via a postjunctional mechanism.

Cite This Study

Liu et al. (1991) studied this question.

synapsesocial.com/papers/6a8bd1192ff0ab4f91e95bf9 https://doi.org/10.1111/j.1476-5381.1991.tb12469.x

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Endogenous nitric oxide modulates adrenergic neural vasoconstriction in guinea‐pig pulmonary artery

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Endogenous nitric oxide modulates adrenergic neural vasoconstriction in guinea‐pig pulmonary artery

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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