Effect of Photodynamic Product Chlorophyllin on Certain Biochemical Parameter in Lymnaea acuminata: Causative Agent of Fasciolosis

Background and Objective: Chlorophyll and chlorophyll derivatives are photosensitizer, which are capable to oxidize and reduce other molecules. The most extensively studied derivative of chlorophyll for their photodynamic activity against insect larvae is chlorophyllin, water-soluble derivative that can be obtained by removal of the phytol tail from the chlorophyll. The aim of present experiment to describe the certain biochemical/enzymatic changes in the gonadal tissues/nervous tissues of Lymnaea acuminata with the treatment of 20 and 60% of 24 h LC 50 of extracted chlorophyllin in sunlight at 24 and 96 h in winter and summer season. Materials and Methods: Chlorophyll was extracted from spinach with the help of macerated leaves for 2 h in 100% ethanol at 55EC. Different biochemical assay estimation with sub-lethal concentration (20 and 60% of 24 h LC 50 ) of chlorophyllin were performed in treated and control group in winter sunlight and summer sunlight. Results: Among all the biochemical parameters the maximum reduction/inhibition was noted in the activity of DNA and AChE against L. acuminata in summer sunlight. Treatment of 60% of 24 h LC 50 of extracted chlorophyllin at 96 h in sunlight caused maximum reduction in DNA (12.19% of control) in summer season. Exposure of 60% of 24 h LC 50 of chlorophyllin for 96 h caused maximum inhibition in enzyme AChE activity (14.36% of control) in nervous tissues of snail in summer sunlight. Conclusion: Maximum variation in DNA and AChE activity by chlorophyllin in the gonadal tissue and nervous tissue of L. acuminata may be responsible for their molluscicidal activity and more effective in summer than winter season (sunlight condition).