Key result
Preincubation with 0.1 μM nicotine maximally promoted GBaSM-4 vascular smooth muscle cell migration toward PDGF-BB (418% relative to control), and 0.1% CSE also significantly enhanced migration.
Why the study?
Does nicotine or cigarette smoke extract promote vascular smooth muscle cell migration toward PDGF-BB in GBaSM-4 cells?
Does nicotine or cigarette smoke extract promote vascular smooth muscle cell migration toward PDGF-BB in GBaSM-4 cells?
Answers are grounded in this paper and cite their sources.
Nicotine and nicotine-free cigarette smoke extract promote vascular smooth muscle cell migration in vitro, suggesting a potential mechanism for VSMC accumulation in atherosclerotic plaques in smokers.
Cigarette smoking is one of the factors causing accumulation of vascular smooth muscle cells (VSMCs) in atherosclerotic plaques. Changes in cell migration toward platelet-derived growth factor BB were investigated using a Boyden chamber after 48-h preincubation of GBaSM-4 VSMCs with nicotine or nicotine-free cigarette smoke extract (CSE). A nicotine concentration of 0.1 µM maximally promoted cell migration; 0.1% CSE also promoted cell migration, while high CSE concentrations damaged GBaSM-4 cells. Fetal bovine serum (FBS) long-depletion induced decrease in migration of GBaSM-4 cells. Our results suggest that nicotine and some CSE components can induce GBaSM-4 cell migration.
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Yoshiyama et al. (2011) studied Atherosclerosis. Nicotine and Cigarette Smoke Extract (CSE) vs. 0 μM nicotine / 0% CSE was evaluated on Relative cell migration toward platelet-derived growth factor BB (PDGF-BB) (418 ± 96% relative migration). Preincubation with 0.1 μM nicotine maximally promoted GBaSM-4 vascular smooth muscle cell migration toward PDGF-BB (418% relative to control), and 0.1% CSE also significantly enhanced migration.
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