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February 6, 2020Frontiers in MicrobiologyOpen Access

Expression, Purification and Characterization of the Hepatitis E Virus Like-Particles in the Pichia pastoris

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Why the study?

Hepatitis E virus causes acute and potentially chronic disease with high mortality in pregnant women, prompting efforts to produce recombinant virus-like particles containing 112-608aa of the ORF2 protein in Pichia pastoris.

Population

BALB/c mice

Comparison

Immunization with Pichia pastoris-produced 112-608aa ORF2 virus-like particles

Design

Preclinical laboratory and animal study

Authors

JGJyoti GuptaIEC UniversitySKSheetal KaulASAkriti SrivastavaCentral University of Karnataka

Discussion

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Implication

Preclinical HEV VLP data support vaccine candidate pursuit; leaves open human immunogenicity and efficacy trials.

Structured PICO

P
Population
BALB/c mice and Pichia pastoris expression system
I
Intervention
Recombinant VLP containing 112-608aa of the ORF2 protein produced in Pichia pastoris
O
Outcome
Immune response (ORF2 specific IgG titer and splenocyte proliferation)surrogate

Recombinant HEV VLPs produced in P. pastoris induce a potent immune response in mice, suggesting potential as a diagnostic and prophylactic candidate.

Cite This Study

Gupta et al. (2020) studied this question.

synapsesocial.com/papers/6a8cfe494847f413e42f0a15https://doi.org/10.3389/fmicb.2020.00141
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Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Essential Elements of the Capsid Protein for Self-Assembly into Empty Virus-Like Particles of Hepatitis E Virus2005 · 142 citations
  2. 2Hepatitis E Virus and Chronic Hepatitis in Organ-Transplant Recipients2008 · 1,357 citations
  3. 3Mutational Analysis of Glycosylation, Membrane Translocation, and Cell Surface Expression of the Hepatitis E Virus ORF2 Protein1999 · 142 citations
  4. 4Genetic heterogeneity of hepatitis E virus*2001 · 328 citations