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November 9, 2022Poultry ScienceOpen Access

Development of a novel competitive ELISA based on nanobody-horseradish peroxidase fusion protein for rapid detection of antibodies against avian hepatitis E virus

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Why the study?

Avian hepatitis E virus causes poultry mortality and economic losses worldwide, but there is no effective serological test for the virus.

Population

An immunized Bactrian camel and clinical chicken serum samples

Comparison

cELISA vs indirect ELISA and Western blot

Design

Diagnostic development and validation study

Authors

TCTianxiang ChenJohns Hopkins UniversityBLBaoyuan LiuBeijing Normal UniversityYCYiyang ChenZhejiang A & F University

Discussion

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Implication

May enable avian HEV serosurveillance in poultry; leaves open prospective validation before routine adoption.

Structured PICO

P
Population
Immunized Bactrian camel and clinical chicken serum samples
I
Intervention
Competitive ELISA (cELISA) using avian HEV-Nb49-HRP fusion protein
C
Comparator
Indirect ELISA and Western blot
O
Outcome
Diagnostic consistency and coincidence (kappa values) with indirect ELISA and Western blotsurrogate

A novel competitive ELISA using a nanobody-HRP fusion protein provides a rapid and reliable method for detecting avian HEV antibodies.

Cite This Study

Chen et al. (2022) studied this question.

synapsesocial.com/papers/6a8cfe77d332c515ad45dcb0https://doi.org/10.1016/j.psj.2022.102326
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Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Identification of an antigenic domain in the N-terminal region of avian hepatitis E virus (HEV) capsid protein that is not common to swine and human HEVs2014 · 8 citations
  2. 2An Egg Drop Associated with Splenomegaly in Broiler Breeders1988 · 53 citations
  3. 3Antigen Specificity and High Affinity Binding Provided by One Single Loop of a Camel Single-domain Antibody2001 · 171 citations
  4. 4Putative Novel Genotype of Avian Hepatitis E Virus, Hungary, 20102012 · 41 citations