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May 9, 2023Open Access

A Multispecies Competitive Nanobody-Based Elisa for the Detection of Antibodies Against Hepatitis E Virus

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Why the study?

Hepatitis E is not easily distinguished clinically from other acute viral hepatitides, creating a need for diagnostic assays to detect and prevent interspecies transmission.

Does a nanobody-based competitive ELISA accurately detect antibodies against Hepatitis E Virus compared to a commercial kit?

Population

Reference panel of human (n = 86) and swine sera (n = 116), plus wild boar, deer, and mice

Comparison

Competitive nanobody-based ELISA vs a commercial kit

Design

Diagnostic validation study

Authors

LALorena Paola ArceMPMaría Florencia PavanMBMarina Bok

Discussion

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Overview

Nanobodies may advance HEV diagnostics in susceptible groups; leaves open clinical validation for transmission prevention.

Structured PICO

Does a nanobody-based competitive ELISA accurately detect antibodies against Hepatitis E Virus compared to a commercial kit?

P
Population
Human (n=86) and swine (n=116) sera reference panel, plus samples from wild boar, deer, and mice
I
Intervention
Competitive ELISA (cELISA) using nanobodies (Nb39 and Nb74) against HEV ORF2
C
Comparator
Commercial kit
O
Outcome
Sensitivity and specificity for detecting anti-HEV antibodiessurrogate

A novel nanobody-based competitive ELISA shows high sensitivity and specificity for detecting anti-HEV antibodies across multiple species.

Cite This Study

Arce et al. (2023) studied this question.

synapsesocial.com/papers/6a7050696ceb2bbd16dfda3chttps://doi.org/10.21203/rs.3.rs-2832762/v1
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Development of a New Multispecies Protein A-Based ELISA for Serodiagnosis of Hepatitis E Infection with Validation in Domestic Swine2024
  2. 2Development of a novel competitive ELISA based on nanobody-horseradish peroxidase fusion protein for rapid detection of antibodies against avian hepatitis E virus2022 · 13 citations
  3. 3Development and Optimization of an Enzyme Immunoassay to Detect Serum Antibodies against the Hepatitis E Virus in Pigs, Using Plant-Derived ORF2 Recombinant Protein2021 · 16 citations
  4. 4ELISA for Antibody to Hepatitis E Virus (HEV) Based on Complete Open-Reading Frame-2 Protein Expressed in Insect Cells: Identification of HEV Infection in Primates1993 · 221 citations
  5. 5Quantitation of Immunoglobulin to Hepatitis E Virus by Enzyme Immunoassay2002 · 51 citations