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September 6, 2021VaccinesOpen Access

Development and Optimization of an Enzyme Immunoassay to Detect Serum Antibodies against the Hepatitis E Virus in Pigs, Using Plant-Derived ORF2 Recombinant Protein

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Why the study?

The study was conducted to develop an enzyme immunoassay using plant-produced recombinant HEV-3 ORF2 to detect anti-hepatitis E virus IgG in pig serum and evaluate its sensitivity and specificity.

Population

Pig serum samples

Comparison

In-house EIA vs commercial assay (PrioCHECK Porcine HEV Ab ELISA Kit)

Design

Assay development and validation study

Authors

KTKaterina TakovaTKTsvetoslav KoynarskiGMG. B. Minkov

Discussion

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Overview

May facilitate swine HEV serosurveillance; leaves open diagnostic utility pending further validation.

Structured PICO

P
Population
Pig serum samples
I
Intervention
In-house enzyme immunoassay (iEIA) using plant-produced recombinant HEV-3 ORF2 110-610_6his capsid protein
C
Comparator
Commercial assay (PrioCHECK™ Porcine HEV Ab ELISA Kit, ThermoFisher Scientific)
O
Outcome
Sensitivity and specificity for the detection of anti-hepatitis E virus IgGsurrogate

A newly developed plant-based enzyme immunoassay can accurately detect anti-HEV IgG in pig serum with high sensitivity and specificity, showing excellent agreement with a commercial kit.

Cite This Study

Takova et al. (2021) studied this question.

synapsesocial.com/papers/6a758c9cfbccc8f4fd160c2dhttps://doi.org/10.3390/vaccines9090991
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Expression, Purification and Characterization of the Hepatitis E Virus Like-Particles in the Pichia pastoris2020 · 20 citations
  2. 2Dimerization of Hepatitis E Virus Capsid Protein E2s Domain Is Essential for Virus–Host Interaction2009 · 133 citations
  3. 3Hepatitis E virus infection during pregnancy2020 · 124 citations
  4. 4Expression in animal cells and characterization of the hepatitis E virus structural proteins1996 · 179 citations
  5. 5Co-expression With Replicating Vector Overcoming Competitive Effects Derived by a Companion Protease Inhibitor in Plants2021 · 7 citations