What question did this study set out to answer?

To describe and validate a surgical technique for permanent left anterior descending artery ligation to establish a standardized mouse model of myocardial infarction.

October 14, 2009Journal of Visualized ExperimentsOpen Access

LAD-Ligation: A Murine Model of Myocardial Infarction

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Population

Laboratory rodents (mice)

Comparison

Permanent ligation of the left anterior… vs Cryo infarction

Design

Preclinical

Authors

MKMandy V.V. KolkUniversity of Veterinary Medicine Vienna DMDanja MeybergUniversität Hamburg TDT. DeuseUniversity of California, San Francisco

Discussion

Member takes

Implication

Supports LAD ligation over cryo-infarction for precise mouse MI models; extends preclinical accuracy while leaving clinical translation open.

Key Points

To describe and validate a surgical technique for permanent left anterior descending artery ligation to establish a standardized mouse model of myocardial infarction.
Demonstration of a surgical protocol in mice to achieve permanent occlusion of the left anterior descending (LAD) artery using a single suture stitch.
Qualitative comparison of the pathophysiological fidelity of LAD ligation against the alternative cryo-infarction method.
LAD ligation induces immediate, visually apparent localized ischemia by preventing blood flow in the target zone while leaving adjacent myocardium largely undamaged.
The technique replicates key pathophysiological features of myocardial infarction and demonstrates high accuracy in correlation with histological sections.

Structured PICO

Population

Laboratory rodents (mice)

Intervention

Permanent ligation of the left anterior descending artery (LAD)

Comparator

Cryo infarction

Outcome

Creation of myocardial ischemia/infarction

LAD ligation in mice is a highly accurate surgical model for studying the pathobiology of myocardial infarction.

Cite This Study

Kolk et al. (2009) studied this question.

synapsesocial.com/papers/6a8cffe18a9f77207c301f48 https://doi.org/10.3791/1438

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Implication

Supports LAD ligation over cryo-infarction for precise mouse MI models; extends preclinical accuracy while leaving clinical translation open.

LAD-Ligation: A Murine Model of Myocardial Infarction

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

LAD-Ligation: A Murine Model of Myocardial Infarction

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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