Key result
N-ethylmaleimide augmented whole-cell currents of KCNQ2, KCNQ4, and KCNQ5 channels by threefold to fourfold by increasing their maximal open probability via alkylation of a cysteine residue in the C terminus.
Effect estimate: threefold to fourfold augmentation
NEM augments KCNQ2, KCNQ4, and KCNQ5 currents by increasing channel open probability via alkylation of specific cysteine residues, rather than altering unitary conductance or membrane trafficking.
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NEM identifies a cysteine-based mechanism to enhance KCNQ currents; leaves open whether this approach can be harnessed therapeutically in neurological disease.
Li et al. (2004) studied KCNQ channel function. N-ethylmaleimide (NEM) vs. Control (before NEM application) was evaluated on Whole-cell current augmentation and maximal open probability (Po) (threefold to fourfold augmentation). N-ethylmaleimide augmented whole-cell currents of KCNQ2, KCNQ4, and KCNQ5 channels by threefold to fourfold by increasing their maximal open probability via alkylation of a cysteine residue in the C terminus.
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