Key result
Microinjection of exogenous cofilin, but not ADF, caused quick disassembly of actin filaments in C2 myoblasts, with its subcellular distribution regulated by phosphorylation at Ser3.
Population
Embryonic chicken skeletal muscle, adult chicken skeletal muscle, mouse C2 myoblasts, and mouse thigh muscle
Comparison
Microinjection of fluorescence-labeled cofilin… vs Control cells without injection
Design
Preclinical
Authors
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Hypothesis-generating for cofilin in muscle actin dynamics; further in vivo studies needed before clinical relevance.
Cofilin is a potent regulator of actin filament reorganization in developing muscle cells, and its activity and subcellular distribution are regulated by phosphorylation.
Obinata et al. (1997) studied Myofibrillogenesis. Cofilin microinjection vs. Control cells without injection or ADF injection was evaluated on Actin filament disassembly and subcellular distribution. Microinjection of exogenous cofilin, but not ADF, caused quick disassembly of actin filaments in C2 myoblasts, with its subcellular distribution regulated by phosphorylation at Ser3.
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