What is the clinical evidence from this study?

Study design: Other. Intervention: Mild alkaline conditions. Primary outcome: Loss of ATPase activity.

What question did this study set out to answer?

To investigate the kinetics and structural mechanisms underlying the loss of ATPase activity in rabbit red skeletal muscle myosin under mild alkaline conditions.

December 1, 1967Journal of Biological ChemistryOpen Access

Studies on Myosin from Red and White Skeletal Muscles of the Rabbit

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Key result

Mild alkaline conditions caused an irreversible loss of ATPase activity in myosin from rabbit red skeletal and cardiac muscles without gross changes in molecular structure.

Population

Myosin from red (slow) and white skeletal muscles, and cardiac muscle of the rabbit

Design

Preclinical

Authors

JSJ.C. SeidelBoston Biomedical Research Institute

Discussion

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Implication

Alerts to myosin ATPase vulnerability in cardiac muscle; leaves open translation to human isoforms or therapies.

Key Points

To investigate the kinetics and structural mechanisms underlying the loss of ATPase activity in rabbit red skeletal muscle myosin under mild alkaline conditions.
Inactivated isolated rabbit red skeletal and cardiac muscle myosin under mild alkaline conditions (pH 9.0) and measured residual ATPase activity over time.
Assessed overall structural integrity and local conformational changes using optical rotatory dispersion, sedimentation analysis, tryptic digestion susceptibility, and sulfhydryl group reactivity assays.
Myosin ATPase inactivation was irreversible and followed two first-order reactions with rate constants of 0.3 min⁻¹ and 0.007 min⁻¹.
Inactivation occurred without major alterations in optical rotatory dispersion or sedimentation, accompanied only by minor increases in tryptic digestion rates and sulfhydryl reactivity near the catalytic site.
Rabbit cardiac muscle myosin lost ATPase activity at pH 9.0 at the same rate as red skeletal muscle myosin.

Structured PICO

Population

Myosin from red (slow) and white skeletal muscles, and cardiac muscle of the rabbit

Exposure

Exposure to mild alkaline conditions (pH 9.0)

Outcome

Loss of ATPase activitysurrogate

The study demonstrates that rabbit cardiac and red skeletal muscle myosin share similar irreversible loss of ATPase activity under mild alkaline conditions without major structural changes.

Cite This Study

J.C. Seidel (1967) studied this question. Mild alkaline conditions was evaluated on Loss of ATPase activity. Mild alkaline conditions caused an irreversible loss of ATPase activity in myosin from rabbit red skeletal and cardiac muscles without gross changes in molecular structure.

synapsesocial.com/papers/6a8e3a76d4d459cac09e1dce https://doi.org/10.1016/s0021-9258(18)99402-0

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Studies on Myosin from Red and White Skeletal Muscles of the Rabbit

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Studies on Myosin from Red and White Skeletal Muscles of the Rabbit

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider