What question did this study set out to answer?

To develop and evaluate a general anion dopant for the negative-ion matrix-assisted laser desorption/ionization mass spectrometry analysis of oligosaccharides.

November 7, 1998Analytical Chemistry

Anion Dopant for Oligosaccharides in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

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Authors

AWAnissa W. WongTheravance Biopharma (United States)MCMark T. CancillaMerck & Co., Inc., Rahway, NJ, USA (United States)LVLisa VossUniversity of California, Davis

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Implication

Experimental study reveals sulfate-mediated negative ionization of neutral oligosaccharides, enabling simultaneous detection of neutral and acidic glycan mixtures in MALDI mass spectrometry.

Key Points

To develop and evaluate a general anion dopant for the negative-ion matrix-assisted laser desorption/ionization mass spectrometry analysis of oligosaccharides.
Doped neutral and acidic oligosaccharides with dilute sulfuric acid solutions (approximately 10^-3 M to 10^-2 M) and analyzed them using negative-ion MALDI mass spectrometry across varying laser fluences.
Mild conditions (approximately 10^-3 M H2SO4 and threshold laser fluence) produced intact [M + HSO4]- sulfate adduct quasimolecular ions for neutral oligosaccharides.
Higher H2SO4 concentrations (approximately 10^-2 M) combined with higher laser fluence caused in situ derivatization, generating [M + HSO4 - H2O]- derivative ions.
Neutral oligosaccharides formed sulfate complexes whereas acidic oligosaccharides underwent deprotonation without adduct formation, enabling the simultaneous detection of both classes in a single mixture.

Cite This Study

Wong et al. (1998) studied this question.

synapsesocial.com/papers/6a9014cbc7b7d4543f89d289 https://doi.org/10.1021/ac980445u

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Implication

Experimental study reveals sulfate-mediated negative ionization of neutral oligosaccharides, enabling simultaneous detection of neutral and acidic glycan mixtures in MALDI mass spectrometry.

Anion Dopant for Oligosaccharides in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Discussion

Member takes

Implication

Key Points

Cite This Study

Anion Dopant for Oligosaccharides in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Discussion

Member takes

Implication

Key Points

Cite This Study

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