Key result
Microchip capillary electrophoresis combined with duplex RT-PCR detected Enterovirus 71 and Coxsackievirus A16 in 100 throat swabs, producing identical results to real-time RT-PCR.
Why the study?
Does microchip capillary electrophoresis with laser-induced fluorescence combined with one-step duplex RT-PCR accurately detect Enterovirus 71 and Coxsackievirus A16 in throat swab specimens compared to real-time RT-PCR?
Does microchip capillary electrophoresis with laser-induced fluorescence combined with one-step duplex RT-PCR accurately detect Enterovirus 71 and Coxsackievirus A16 in throat swab specimens compared to real-time RT-PCR?
The developed microchip capillary electrophoresis method provides a rapid, sensitive, and specific alternative to real-time RT-PCR for detecting Enterovirus 71 and Coxsackievirus A16.
Answers are grounded in this paper and cite their sources.
May offer rapid alternative for EV71/CA16 detection; leaves open larger validation before clinical adoption.
Enterovirus 71 and Coxsackievirus A16 are the main pathogens causing hand-foot-mouth disease. In this paper, microchip capillary electrophoresis with laser-induced fluorescence combined with one-step duplex reverse transcript-polymerase chain reaction has been developed for the detection of Enterovirus 71 and Coxsackievirus A16 in throat swab specimens. The specific reverse transcription-polymerase chain reaction amplicons labeled with SYBR Orange were separated by microchip capillary electrophoresis and detected by laser induced fluorescence detector within 7 min. The intraday and interday relative standard deviation of migration time for DNA Marker was in the range of 1.36-2.94 and 2.78-3.96%, respectively. The detection limits were as low as 2.06 × 10(3) copies/mL for Enterovirus 71 and 5 × 10(3) copies/mL for Coxsackievirus A16. No cross-reactivity was observed with rotavirus, astrovirus, norovirus, and adenovirus, which showed good specificity of the method. This assay was validated using 100 throat swab specimens that were detected by real-time reverse-transcript polymerase chain reaction in parallel and the two methods produced the same results. This study provided a rapid, sensitive and specific method for the detection of Enterovirus 71 and Coxsackievirus A16, which make a contribution to significant time and cost saving for the identification and treatment of patients.
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Ruan et al. (2015) studied Hand-foot-mouth disease (Enterovirus 71 and Coxsackievirus A16) (n=100). Microchip capillary electrophoresis with laser-induced fluorescence combined with one-step duplex RT-PCR vs. Real-time reverse-transcript polymerase chain reaction was evaluated on Concordance of detection results with real-time RT-PCR. Microchip capillary electrophoresis combined with duplex RT-PCR detected Enterovirus 71 and Coxsackievirus A16 in 100 throat swabs, producing identical results to real-time RT-PCR.
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