What question did this study set out to answer?

To evaluate whether late pregnancy alters the function of perivascular sympathetic and nitrergic nerves in mesenteric arteries and to identify the underlying physiological mechanisms.

May 7, 2015PLoS ONEOpen Access

Alterations in Perivascular Sympathetic and Nitrergic Innervation Function Induced by Late Pregnancy in Rat Mesenteric Arteries

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Why the study?

Does late pregnancy alter perivascular sympathetic and nitrergic innervation function in rat mesenteric arteries?

Population

Female Sprague-Dawley rats (4-6 months old)

Comparison

Late pregnancy (20 days of pregnancy) vs Control (virgin females in oestrous phase)

Design

Preclinical

Authors

ESEsther SastreComplejo Asistencial Universitario de Palencia JBJavier Blanco‐RiveroHospital La Paz Institute for Health Research LCLaura CaracuelHospital Universitario La Paz

Discussion

Member takes

Implication

Provides mechanistic insight in pregnant rats; leaves open translation to human pregnancy and clinical practice.

Key Points

To evaluate whether late pregnancy alters the function of perivascular sympathetic and nitrergic nerves in mesenteric arteries and to identify the underlying physiological mechanisms.
Harvested superior mesenteric arteries from female Sprague-Dawley rats divided into non-pregnant controls (in oestrous phase) and late-pregnant rats (day 20 of gestation).
Assessed vasoconstrictor responses to electrical field stimulation (EFS) with or without phentolamine or L-NAME, and measured vasomotor reactivity to noradrenaline and the nitric oxide donor DEA-NO.
Quantified noradrenaline and nitric oxide release and analyzed neuronal nitric oxide synthase (nNOS) expression and activation levels.
EFS-induced vasoconstriction was significantly lower across stimulation frequencies in pregnant rats compared with non-pregnant controls.
Phentolamine decreased EFS-induced contractions more markedly in controls, whereas L-NAME caused a greater enhancement of constriction in pregnant rats.
Late pregnancy decreased noradrenaline release and noradrenaline-mediated vasoconstriction, while enhancing nitric oxide release and nNOS activation without altering total nNOS expression or DEA-NO-induced vasodilation.

Structured PICO

Does late pregnancy alter perivascular sympathetic and nitrergic innervation function in rat mesenteric arteries?

Population

Female Sprague-Dawley rats (4-6 months old)

Intervention

Late pregnancy (20 days of pregnancy)

Comparator

Control (virgin females in oestrous phase)

Outcome

Vasoconstrictor response to electrical field stimulation (EFS)surrogate

Late pregnancy decreases mesenteric vasoconstriction to electrical field stimulation by diminishing sympathetic noradrenaline release and enhancing nitrergic nitric oxide release.

Cite This Study

Sastre et al. (2015) studied this question.

synapsesocial.com/papers/6a908c30e02ba012352dc867 https://doi.org/10.1371/journal.pone.0126017

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Alterations in Perivascular Sympathetic and Nitrergic Innervation Function Induced by Late Pregnancy in Rat Mesenteric Arteries

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Alterations in Perivascular Sympathetic and Nitrergic Innervation Function Induced by Late Pregnancy in Rat Mesenteric Arteries

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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