What question did this study set out to answer?

To determine whether human platelet concentrates stored at 22°C retain their functional aggregation and secretory responses when exposed to synergistic pairs of aggregating agents.

March 1, 1982Blood

Stored human platelets retain full aggregation potential in response to pairs of aggregating agents

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To determine whether human platelet concentrates stored at 22°C retain their functional aggregation and secretory responses when exposed to synergistic pairs of aggregating agents.
Assessed eight platelet concentrates (N=8) stored at 22°C on the day of preparation and after 3 and 5 days of storage.
Measured platelet aggregation and secretion using a lumi-aggregometer and quantified thromboxane-B2 formation using radioimmunoassay across single and paired agonist conditions (ADP, epinephrine, collagen, and arachidonic acid).
Platelets stored for 5 days became completely unresponsive to single epinephrine, markedly insensitive to ADP, and moderately insensitive to collagen and arachidonic acid.
Paired aggregating agents (such as 2 µM ADP combined with 2 µM epinephrine, or either combined with low concentrations of collagen or arachidonic acid) produced no statistically significant loss of aggregation or secretion sensitivity after 5 days of storage.
Thromboxane-B2 synthesis decreased under single-agent stimulation after 5 days of storage, but remained unchanged compared to fresh platelets when challenged with low-dose pairs of agents.

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Implication

In vitro study demonstrates preserved aggregation in stored human platelet concentrates exposed to paired agonists, suggesting why stored platelets function effectively in vivo.

Cite This Study

DiMinno et al. (1982) studied this question.

synapsesocial.com/papers/6a90fe6f152a692a9d92de66 https://doi.org/10.1182/blood.v59.3.563.563

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Implication

In vitro study demonstrates preserved aggregation in stored human platelet concentrates exposed to paired agonists, suggesting why stored platelets function effectively in vivo.

Stored human platelets retain full aggregation potential in response to pairs of aggregating agents

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Discussion

Implication

Cite This Study

Stored human platelets retain full aggregation potential in response to pairs of aggregating agents

Key Points

Discussion

Implication

Cite This Study

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