October 1, 1997Journal of Biological ChemistryOpen Access

Dantrolene Inhibition of Sarcoplasmic Reticulum Ca2+Release by Direct and Specific Action at Skeletal Muscle Ryanodine Receptors

View Full Paper

Ask AI

Bookmark

Why the study?

Does dantrolene directly inhibit ryanodine receptor Ca2+ channel function in isolated sarcoplasmic reticulum vesicles?

Population

Isolated sarcoplasmic reticulum vesicles prepared from malignant hyperthermia-susceptible and normal pig…

Comparison

Dantrolene vs Untreated conditions / cardiac sarcoplasmic…

Design

Preclinical

Authors

BFBradley R. FruenUniversity of North Carolina at Chapel Hill JMJames R. MickelsonWashington State University CLCharles F. LouisMichigan State University

Discussion

Member takes

Implication

Hypothesis-generating for dantrolene's skeletal RyR selectivity; leaves open cardiac safety in humans.

Structured PICO

Does dantrolene directly inhibit ryanodine receptor Ca2+ channel function in isolated sarcoplasmic reticulum vesicles?

Population

Isolated sarcoplasmic reticulum vesicles prepared from malignant hyperthermia-susceptible and normal pig skeletal muscle, purified skeletal muscle ryanodine receptor protein reconstituted into liposomes, and cardiac sarcoplasmic reticulum vesicles

Intervention

Dantrolene

Comparator

Untreated conditions / cardiac sarcoplasmic reticulum vesicles

Outcome

45Ca2+ release half-time and [3H]ryanodine bindingsurrogate

Dantrolene selectively inhibits skeletal muscle ryanodine receptors by directly limiting their activation by calmodulin and Ca2+, without affecting cardiac ryanodine receptors.

Cite This Study

Fruen et al. (1997) studied this question.

synapsesocial.com/papers/6a912e26443e712ea3d2c284 https://doi.org/10.1074/jbc.272.43.26965

View Full Paper

Ask AI

Bookmark

Dantrolene Inhibition of Sarcoplasmic Reticulum Ca2+Release by Direct and Specific Action at Skeletal Muscle Ryanodine Receptors

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Dantrolene Inhibition of Sarcoplasmic Reticulum Ca2+Release by Direct and Specific Action at Skeletal Muscle Ryanodine Receptors

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider