Enamel Remineralisation and Antimicrobial Efficacy of a Withania somnifera, Ficus religiosa, Salvia rosmarinus-based Polyherbal Gel: A Research Protocol
In vitro study protocol reports testing of a polyherbal gel against oral pathogens on extracted human teeth, suggesting safer natural strategies for enamel remineralisation.
Key Points
To evaluate the antimicrobial, antibiofilm, and enamel remineralisation efficacy of a polyherbal gel formulated from Withania somnifera, Ficus religiosa, and Salvia rosmarinus compared with casein phosphopeptide-amorphous calcium phosphate (CPP-ACP).
In-vitro experimental protocol planned at Datta Meghe Institute of Higher Education and Research, India, from December 2025 to December 2026, utilizing 70 extracted human teeth (orthodontic or non-cavitated lesions) randomly allocated into two equal arms (n=35 each): polyherbal gel and CPP-ACP control.
Antimicrobial potency against Streptococcus mutans and Lactobacillus species will be quantified via agar diffusion and broth microdilution assays, alongside biofilm inhibition and disruption evaluations.
Enamel remineralisation will be visualized and assessed using scanning electron microscopy, with intra-group and inter-group comparisons evaluated by paired and independent t-tests (significance at p≤0.05).
This is a formal research protocol; prospective comparative event rates, relative effect sizes, confidence intervals, and p-values will be recorded upon completion of in-vitro experiments.
Primary planned endpoints will report quantitative differences in bacterial growth zones, percentage reduction in biofilm mass, and surface mineral density between the polyherbal gel and CPP-ACP.