Key result
The developed recombinant antigen-based indirect ELISA demonstrated a diagnostic specificity of 99.47% and a sensitivity of 92% compared to a commercial kit, with high agreement (k-criterion 0.88).
Effect estimate: k-criterion 0.88
A newly developed recombinant antigen-based indirect ELISA for porcine epidemic diarrhea virus demonstrates high specificity and sensitivity, offering a reliable diagnostic tool for veterinary use.
Should not yet alter diagnostic practice; leaves open need for field validation in target populations.
Spread of porcine epidemic diarrhea and its increased threat for the pig industry necessitate development of advanced techniques for the disease diagnosis. Use of recombinant antigens of the disease agent seems prospective. The recombinant antigen-based indirect enzyme-linked immunosorbent assay has been developed for the detection of antibodies against porcine epidemic diarrhea virus. The research performed allowed for determination of all necessary test conditions. Basic features of the developed method were determined during its validation. The test precision was above 90% pursuant to its repeatability and reproducibility, diagnostic specificity of the test amounted to 99.47%, and its sensitivity as compared to commercial ID Screen PEDV Indirect (IDvet, France) was 92% with very good compatibility of the two tests (k-criterion – 0.88) – 92%. The test-system demonstrates high stability upon the change of the key test component.
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Kanshina et al. (2018) studied Porcine epidemic diarrhea (n=1,610). Recombinant antigen-based indirect ELISA vs. Commercial ID Screen PEDV Indirect (IDvet, France) was evaluated on Diagnostic sensitivity and specificity (k-criterion 0.88). The developed recombinant antigen-based indirect ELISA demonstrated a diagnostic specificity of 99.47% and a sensitivity of 92% compared to a commercial kit, with high agreement (k-criterion 0.88).
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