Key result
The developed S1 indirect ELISA demonstrated an overall coincidence rate of 96.74% with IFA, with 96.71% sensitivity and 96.77% specificity for detecting PEDV antibodies in swine serum.
The developed S1 indirect ELISA is a highly sensitive and specific method for the serological evaluation and indirect diagnosis of PEDV infection in swine.
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S1 ELISA shows high agreement with IFA for PEDV serology in swine; supports further validation before routine diagnostic adoption.
Lin et al. (2018) studied Porcine epidemic diarrhea virus (PEDV) infection (n=3,672). S1 indirect ELISA vs. Indirect immunofluorescent assay (IFA) was evaluated on Overall coincidence rate with IFA. The developed S1 indirect ELISA demonstrated an overall coincidence rate of 96.74% with IFA, with 96.71% sensitivity and 96.77% specificity for detecting PEDV antibodies in swine serum.
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