What question did this study set out to answer?

To evaluate the use of the alpha-glucan-uridine diphosphate glucosyltransferase reaction to increase visual contrast between glycogen-depleted and non-depleted muscle fibers.

September 1, 1978Journal of Histochemistry & CytochemistryOpen Access

The alpha-glucan-uridine diphosphate glucosyltransferase reation for the identification of glycogen-depleted muscle fibers.

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Authors

CPC.W. PoolBoston University YDY. E. Donselaar PGPaul A. M. GriepKempenhaeghe

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Implication

Laboratory study demonstrates enhanced histological contrast of glycogen-depleted fibers in rat skeletal muscle, improving the visual identification of activated motor units.

Key Points

To evaluate the use of the alpha-glucan-uridine diphosphate glucosyltransferase reaction to increase visual contrast between glycogen-depleted and non-depleted muscle fibers.
Depleted glycogen in muscle fibers through prolonged repetitive electrical stimulation of single motor units in the rat extensor digitorum longus muscle.
Applied the alpha-glucan-uridine diphosphate glucosyltransferase reaction coupled with periodic acid-Schiff (PAS) staining to analyze muscle tissue sections.
The enzymatic reaction enhanced the visual contrast between glycogen-depleted and non-depleted muscle fibers in the PAS reaction (quantitative metrics not reported).

Cite This Study

Pool et al. (1978) studied this question.

synapsesocial.com/papers/6a91aa535a186543ed935cff https://doi.org/10.1177/26.9.712050

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Implication

Laboratory study demonstrates enhanced histological contrast of glycogen-depleted fibers in rat skeletal muscle, improving the visual identification of activated motor units.

The alpha-glucan-uridine diphosphate glucosyltransferase reation for the identification of glycogen-depleted muscle fibers.

Discussion

Member takes

Implication

Key Points

Cite This Study

The alpha-glucan-uridine diphosphate glucosyltransferase reation for the identification of glycogen-depleted muscle fibers.

Discussion

Member takes

Implication

Key Points

Cite This Study

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