Myocardial Cytoskeletal Adaptations in Advanced Kidney Disease

Figures

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  Figure 1. The Cardiac Aging in CKD (CAIN) Cohort and Study Protocol. A total of 109 deceased donor participants have been recruited to‐date in the CAIN cohort. Patients in the hypertension (HTN) group were included based on a documented history of diagnosed hypertension. All donor hearts underwent quality control (QC) screening for RNA purity. RNA quality was determined by nucleic acids absorbance ratio by spectrophotometry and a ratio ≈2.0 was considered as high purity. One patient was excluded due to poor RNA quality. Strict exclusion criteria for each group were used as illustrated. A total of 50 participants were included in the final analysis. CKD, chronic kidney disease; GFR, glomerular filtration rate; LVH, left ventricular hypertrophy.

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  Figure 2. Cardiac characterization. A, Gross pathologic characterization. All heart samples underwent gross pathologic examination. Hemodialysis (HD) heart weight in grams (i) and normalized to body surface area (BSA) (ii) was significantly greater in HD and hearts from the hypertension group (HTN). HD and HTN hearts had increased left ventricular wall thickness (iii) expressed in centimeters (cm) and (iv) normalized to BSA. While HD and HTN hearts appeared to have increased left atrial area (v) expressed in cm2, this was not statistically significant following normalization to BSA (vi). Box‐and‐whisker plot denote extremes, interquartile range, and median. Displayed data points include outliers. P value was obtained by ANOVA for 3‐group comparison. Post hoc analysis by Tukey‐Kramer Honestly Significant Differences (HSD)test. B, Masson's trichrome stain of fibrotic changes in HD and HTN heart tissues: Left ventricular tissue sections were stained with Masson’s trichome stain. Left panels: 500 µm scale; Right panels: 100 µm scale of enlarged areas shown in the yellow boxes in the top panel. Left ventricular tissues from HD participants had severe and widespread fibrosis compared with…

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  Figure 3. Transcriptomic profiles of heart tissues, ex vivo. A, RNA‐seq analysis of heart tissues (left ventricular [LV] free wall) comparing hemodialysis (HD) and control groups. Top 26 significantly differentially expressed genes are highlighted in red (false discovery rate [FDR] adjusted P values <0.05). The x‐axis denotes mean log counts per million (log2CPM) and the y‐axis denotes log fold change (log2FC). B, Transcriptomics pathway enrichment analysis. Approximately 300 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways are aligned in the order of statistical significance. C, Gene expression ratios mapped to the focal adhesion pathway and pseudo‐colored according to the indicated scale. Genes with blank rectangles had insufficient counts. Dotted lines denote indirect effect. CPM indicates counts per million; and FC, fold‐change.

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  Figure 4. Dysregulation of the cytoskeleton is a cardinal feature of myocardial failure in patients undergoing hemodialysis, ex vivo. A, Cytoskeletal genes are significantly altered in hemodialysis (HD) hearts. mRNA expression of major cytoskeletal proteins β‐actin, β‐tubulin, vimentin and vinculin as assessed by quantitative polymerase chain reaction (PCR). β‐actin, β‐tubulin, and vimentin was significantly dysregulated in hearts from patients undergoing hemodialysis compared with control. Hypertensive (HTN) hearts had dysregulation of β‐actin and β‐tubulin, but not vimentin compared with control. GAPDH was used as a standard. B, Cytoskeletal protein expressions in left ventricular heart tissues. (i) β‐actin, (ii) β‐tubulin, and (iii) vimentin expression was significantly suppresed in HD hearts. However, only β‐tubulin and vimentin expression was reduced in HTN hearts; (iv) vinculin expression was significantly upregulated in HD hearts but not in HTN hearts. (*P<0.05; **P<0.01).

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  Figure 5. Disruption of mitochondrial bioenergetics and cell survival pathways occurs in the failing myocardium in patients undergoing hemodialysis, ex vivo. quantitative polymerase chain reaction was used to assess mRNA levels of genes identified on RNA sequencing related to mitochondrial function, cell survival and apoptosis. A, Mitochondrial function is significantly altered in hearts from HD and patients with HTN: Mitochondrial function, reflected by expression of OPA1 and MFN1, critical genes that regulate mitochondrial division and fusion activities was severely suppressed in both hearts from HD and patients with HTN. B, Pro‐apoptotic pathways are activated in hearts from HD and patients with HTN. In HD hearts, caspase 3, as well as P53, cytochrome C (CYCS) and BAX was upregulated. In HTN hearts, caspase 3 was downregulated but expression of P53, CYCS and BAX was increased. C, Anti‐apoptotic genes are suppressed in HD and HTN hearts. Anti‐apoptotic genes, BCL2 and BAG1 were down‐regulated in both HD and HTN hearts. (*:P<0.05; **:P<0.01).

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  Figure 6. Cytoskeletal dysregulation can be driven by uremic stress, in vitro. Primary human cardiac fibroblasts were cultured from the ventricle. Expression of target proteins was assesed by immunoblotting. A, Dysregulation of all major cytoskeletal compartments following treatment with pooled uremic serum (US). Cardiac fibroblasts were treated with 10% pooled human uremic serum or 10% human serum from healthy donors for 48 hours. (i) β‐actin, (ii) β‐tubulin, and (iii) vimentin was significantly suppresed in cardiac fibroblasts from the ventricle following uremic treatment. Vinculin (iv) expression did not significantly change with uremic treatment. B, Dysregulation of all major cytoskeletal compartments following treatment with TNF‐α. Cardiac fibroblasts were treated with TNF‐α at 20 ng/mL for 48 hours. (i) β‐actin, (ii) β‐tubulin, (iii) vimentin, and (iv) vinculin were all suppressed in cardiac fibroblasts from the ventricle following treatment. C, Cytoskeletal dysregulation can be driven by uremic mineral stress. β‐actin (i) was significantly suppressed with either phosphate or calcium treatments, but not with combined phosphate and calcium treatment. β‐tubulin (ii) was significantly…

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  Figure 7. Regulation of the cardiac cytoskeleton by focal adhesion kinase (FAK). A, Expression of FAK mRNA is significantly upregulated in left ventricular tissues from patients undergoing hemodialysis, ex vivo. Analysis of FAK mRNA expression by quantitative polymerase chain reaction revealed that FAK was significantly upregulated in hearts from patients undergoing hemodialysis. However, FAK mRNA expression was suppressed in hearts from patients with HTN. B, Expression of FAK protein is upregulated in left ventricular tissues from patients undergoing hemodialysis, ex vivo. Protein expression of FAK was significantly upregulated in hearts from patients undergoing hemodialysis as assessed by immunoblotting. FAK protein expression was significantly suppressed in HTN hearts. C, FAK silencing mediates cytoskeletal dysfunction in the presence of mineral stressors. (i) Primary human ventricular cardiac fibroblasts were transfected once with either scrambled or FAK siRNA. Cells were then treated daily with or without mineral stressors (ie P+Ca: 2 mmol/L β‐glycerophosphate with 1 unit/mL alkaline phosphatase, and 2.7 mmol/L calcium chloride) for 5 days. After treatment, target cytoskeletal…