What question did this study set out to answer?

To determine whether atrial natriuretic factor requires an increase in glomerular filtration rate to induce natriuresis and diuresis.

October 1, 1985AJP Renal Physiology

Effects of synthetic atrial natriuretic factor in the isolated perfused rat kidney

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Why the study?

Does synthetic atrial natriuretic factor induce natriuresis and diuresis independent of increases in glomerular filtration rate in isolated perfused rat kidneys?

Population

Isolated perfused rat kidneys

Comparison

Atrial extracts or synthetic atrial natriuretic… vs Control levels (baseline or post-infusion)

Design

Preclinical

Authors

RMRobert MurrayUniversity of Leeds SISadayoshi ItoVascular Medicine TITadashi InagamiHeart Failure / Cardiomyopathy

Discussion

Member takes

Implication

Suggests tubular ANF action in rat kidneys; leaves open translation to clinical use.

Key Points

To determine whether atrial natriuretic factor requires an increase in glomerular filtration rate to induce natriuresis and diuresis.
Perfused isolated rat kidneys with atrial tissue extracts (1 mg/ml) or synthetic atrial natriuretic factor (3, 31, and 61 ng/ml) at constant pressure in a single-pass system.
Measured glomerular filtration rate, electrolyte excretion (sodium and potassium), and water excretion during continuous infusions and after infusion cessation.
High-dose synthetic atrial natriuretic factor (31 and 61 ng/ml) and atrial extracts significantly increased glomerular filtration rate, electrolyte excretion, and water excretion, with sodium and water excretion remaining elevated after infusion stopped.
Low-dose synthetic atrial natriuretic factor (3 ng/ml) significantly increased sodium and water excretion without altering glomerular filtration rate or potassium excretion.
Potassium excretion increased only under experimental conditions where glomerular filtration rate was also elevated.

Structured PICO

Does synthetic atrial natriuretic factor induce natriuresis and diuresis independent of increases in glomerular filtration rate in isolated perfused rat kidneys?

Population

Isolated perfused rat kidneys

Intervention

Atrial extracts (1 mg/ml) or synthetic atrial natriuretic factor (ANF) at high (31 and 61 ng/ml) or low (3 ng/ml) concentrations infused at constant pressure in a single-pass system

Comparator

Control levels (baseline or post-infusion)

Outcome

Glomerular filtration rate (GFR) and electrolyte (sodium, potassium) and water excretionsurrogate

Synthetic atrial natriuretic factor induces natriuresis and diuresis independently of increases in glomerular filtration rate in isolated rat kidneys.

Cite This Study

Murray et al. (1985) studied this question.

synapsesocial.com/papers/6a92327d3da4caa5ce963289 https://doi.org/10.1152/ajprenal.1985.249.4.f603

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Effects of synthetic atrial natriuretic factor in the isolated perfused rat kidney

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Effects of synthetic atrial natriuretic factor in the isolated perfused rat kidney

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider