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February 1, 1989Biochemistry

Binding of myosin to actin in myofibrils during ATP hydrolysis

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Population

myofibrils

Design

Preclinical

Authors

ADAnh M. DuongUniversity of California, Los AngelesEREmil ReislerUniversity of California, Los Angeles

Discussion

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Implication

May refine cross-bridge models; leaves open applicability to intact human cardiac muscle.

Structured PICO

P
Population
myofibrils
I
Intervention
cross-linking with 10 mM carbodiimide (EDC) under rigor conditions at 4 degrees C, followed by tryptic and chymotryptic digestion
O
Outcome
binding of myosin to actin in myofibrils during ATP hydrolysissurrogate

Demonstrates that approximately 25% of myosin heads exhibit rigor-like binding to actin in myofibrils during ATP hydrolysis.

Cite This Study

Duong et al. (1989) studied this question.

synapsesocial.com/papers/6a925201ad37002f23384a0ahttps://doi.org/10.1021/bi00429a054
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1The binding of myosin heads on heavy meromyosin and assembled myosin to actin in the presence of nucleotides. Measurements by the proteolytic rates method.1987 · 10 citations
  2. 2A structural and kinetic study on myofibrils prevented from shortening by chemical cross-linking1993 · 46 citations
  3. 3Identification of myosin-binding sites on the actin sequence1982 · 327 citations
  4. 4A Myosin Head Can Interact with Two Chemically Modified G-Actin Monomers at ATP-Modulated Multiple Sites1996 · 4 citations
  5. 5Tryptic digestion of rabbit skeletal myofibrils: an enzymatic probe of myosin cross-bridges1984 · 22 citations