Key result
Two moles of MBS-actin monomers bound to 1 mol of myosin heads or S1 with different affinities, protecting specific junctions from trypsin digestion, which was weakened by ATP.
The study demonstrates that a single myosin head can interact with two chemically modified G-actin monomers at multiple sites, which are modulated by ATP.
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No clinical implications yet; leaves open how dual actin binding modulates cardiac myosin in vivo.
Toshiaki Arata (1996) studied this question. MBS-G-actin and myosin subfragment 1 (S1) was evaluated on Binding and cross-linking of MBS-G-actin to S1. Two moles of MBS-actin monomers bound to 1 mol of myosin heads or S1 with different affinities, protecting specific junctions from trypsin digestion, which was weakened by ATP.
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