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Investigate the chemical identity of endothelium-derived relaxing factor in cerebral microvessels and evaluate how nitric oxide synthesis inhibitors affect acetylcholine-induced vasodilation.

November 1, 1992StrokeOpen Access

Effects in cats of inhibition of nitric oxide synthesis on cerebral vasodilation and endothelium-derived relaxing factor from acetylcholine.

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Investigate the chemical identity of endothelium-derived relaxing factor in cerebral microvessels and evaluate how nitric oxide synthesis inhibitors affect acetylcholine-induced vasodilation.
Measured cerebral arteriolar caliber and bioassayed endothelium-derived relaxing factor production in anesthetized cats using cranial windows during acetylcholine superfusion (10⁻⁷ M).
Tested responses before and after administering nitric oxide synthesis inhibitors NG-monomethyl L-arginine and NG-nitro-L-arginine, with permeability alteration via sodium dodecyl sulfate and reversal testing via L-arginine.
NG-nitro-L-arginine completely abolished acetylcholine-induced arteriolar vasodilation and eliminated endothelium-derived relaxing factor production, an effect reversed by L-arginine.
NG-monomethyl L-arginine exerted identical inhibitory effects only following cell membrane permeabilization with sodium dodecyl sulfate.
Inhibitors did not change baseline vascular caliber, produce vasoconstrictor agents, or alter responses to nitroprusside, adenosine, superoxide dismutase, or catalase.

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Implication

Experimental animal study demonstrates that nitric oxide synthesis inhibitors block acetylcholine-induced arteriolar dilation in cats, indicating endothelium-derived relaxing factor is nitric oxide.

Cite This Study

Wei et al. (1992) studied this question.

synapsesocial.com/papers/6a92575f5ab1441fe3c811eb https://doi.org/10.1161/01.str.23.11.1623

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Implication

Experimental animal study demonstrates that nitric oxide synthesis inhibitors block acetylcholine-induced arteriolar dilation in cats, indicating endothelium-derived relaxing factor is nitric oxide.

Effects in cats of inhibition of nitric oxide synthesis on cerebral vasodilation and endothelium-derived relaxing factor from acetylcholine.

Key Points

Discussion

Implication

Cite This Study

Effects in cats of inhibition of nitric oxide synthesis on cerebral vasodilation and endothelium-derived relaxing factor from acetylcholine.

Key Points

Discussion

Implication

Cite This Study

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