What question did this study set out to answer?

To determine whether a high-fat/high-carbohydrate diet without added cholesterol impairs aortic valve function and alters leaflet morphology in wild-type and LDL receptor-deficient mice.

January 27, 2006Journal of the American College of CardiologyOpen Access

A High Fat/High Carbohydrate Diet Induces Aortic Valve Disease in C57BL/6J Mice

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Why the study?

Does a high-fat/high-carbohydrate diet induce aortic valve disease in wild-type and LDLr-/- mice?

Population

40 adult C57BL/6J mice (wild-type and low-density lipoprotein receptor-deficient [LDLr-/-])

Comparison

High-fat/high-carbohydrate diet for 4 months vs Normal diet

Design

Preclinical

Follow-up

4 months

Authors

MDMarie‐Claude DroletInstitut Universitaire de Cardiologie et de Pneumologie de Québec ÉRÉlise RousselUniversité de Sherbrooke YDYves DeshaiesUniversité Laval

Discussion

Member takes

Implication

HF/HC diet induces aortic valve changes in WT mice; hypothesis-generating for metabolic drivers of stenosis beyond hypercholesterolemia.

Key Points

To determine whether a high-fat/high-carbohydrate diet without added cholesterol impairs aortic valve function and alters leaflet morphology in wild-type and LDL receptor-deficient mice.
Divided 40 C57BL/6J mice into four experimental groups: wild-type on a normal diet, wild-type on a high-fat/high-carbohydrate (HF/HC) diet, LDLr-/- on a normal diet, and LDLr-/- on an HF/HC diet.
Evaluated aortic valve function and leaflet histology using echocardiography and tissue analysis after four months of dietary intervention.
Wild-type mice on the HF/HC diet developed obesity, hyperglycemia, and mild hypercholesterolemia, whereas LDLr-/- mice developed severe hypercholesterolemia.
Both wild-type and LDLr-/- mice fed the HF/HC diet displayed smaller valve areas and significantly higher transvalvular velocities (p < 0.01) after four months.
Aortic valve leaflets in both HF/HC-fed groups became thickened and showed extensive infiltration by lipids and macrophages.

Structured PICO

Does a high-fat/high-carbohydrate diet induce aortic valve disease in wild-type and LDLr-/- mice?

Population

40 adult C57BL/6J mice (wild-type and low-density lipoprotein receptor-deficient [LDLr-/-])

Intervention

High-fat/high-carbohydrate (HF/HC) diet for 4 months

Comparator

Normal diet

Outcome

Aortic valve function and morphology (valve area, transvalvular velocities, histology) evaluated by echocardiography after four monthssurrogate

A high-fat/high-carbohydrate diet induces aortic valve abnormalities in wild-type mice, demonstrating that metabolic factors beyond severe hypercholesterolemia can drive aortic stenosis.

Cite This Study

Drolet et al. (2006) studied this question.

synapsesocial.com/papers/6a925f6b9d072e6712f0cdfe https://doi.org/10.1016/j.jacc.2005.09.049

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A High Fat/High Carbohydrate Diet Induces Aortic Valve Disease in C57BL/6J Mice

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

A High Fat/High Carbohydrate Diet Induces Aortic Valve Disease in C57BL/6J Mice

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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