Key result
Synthetic peptides P-3 (third intracellular loop) and P-5 (C-terminal tail) of the rat AT1A receptor activated purified Gi1, Gi2, and Go proteins, indicating their importance for G protein coupling.
The third cytosolic loop and C-terminal cytosolic domain of the rat AT1A receptor are critical for coupling and activating Gi1, Gi2, and Go proteins.
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Does not alter clinical AT1 blockade; extends in vitro mapping of rodent receptor-G protein domains but leaves translation open.
Shirai et al. (1995) studied this question. Synthetic peptides representing domains of cytosolic segments of the rat AT1A receptor was evaluated on Activation of purified Gi1, Gi2, and Go proteins (binding of 35S-labeled GTP gamma S). Synthetic peptides P-3 (third intracellular loop) and P-5 (C-terminal tail) of the rat AT1A receptor activated purified Gi1, Gi2, and Go proteins, indicating their importance for G protein coupling.
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