Key result
A mammalian splicing commitment complex was partially purified and shown to be a required intermediate for complex A formation, requiring U1 snRNP, splice sites, and a polypyrimidine tract.
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Hypothesis-generating for spliceosome assembly; leaves open relevance to cardiac splicing defects.
Jamison et al. (1992) studied this question. In vitro spliceosome assembly assays was evaluated on Spliceosome commitment complex formation and chase to complex A and B. A mammalian splicing commitment complex was partially purified and shown to be a required intermediate for complex A formation, requiring U1 snRNP, splice sites, and a polypyrimidine tract.
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