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In this tutorial, we discuss the motivations for doing two-dimensional liquid chromatography (2D-LC) and describe the commonly used implementations of the method. We review important guiding principles for method development, discuss the state of the art in 2D-LC performance as measured by peak capacity, and describe example applications from different fields that we hope will inspire new users to adopt 2D-LC for their analytical problems.
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Stoll et al. (2016) studied this question.
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