Key result
The modified loaded heterotopic heart transplant model significantly increased pulmonary artery diameter (0.62 vs 0.44 mm, p=0.0367) and right ventricular stroke volume compared to the traditional unloaded model.
Why the study?
Does a modified loaded heterotopic heart transplant model in mice improve hemodynamic function and allow for the study of neotissue formation in decellularized pulmonary valves compared to unloaded models?
Does a modified loaded heterotopic heart transplant model in mice improve hemodynamic function and allow for the study of neotissue formation in decellularized pulmonary valves compared to unloaded models?
Absolute Event Rate: 0.62% vs 0.44%
p-value: p=0.0367
This study establishes a modified loaded murine heterotopic heart transplant model that better simulates physiological hemodynamics for investigating neotissue formation in tissue-engineered heart valves.
Should not yet alter clinical valve practices; extends murine platforms for loaded neotissue studies.
Decellularized allograft heart valves have been used as tissue-engineered heart valve (TEHV) scaffolds with promising results; however, little is known about the cellular mechanisms underlying TEHV neotissue formation. To better understand this phenomenon, we developed a murine model of decellularized pulmonary heart valve transplantation using a hemodynamically unloaded heart transplant model. Furthermore, because the hemodynamics of blood flow through a heart valve may influence morphology and subsequent function, we describe a modified loaded heterotopic heart transplant model that led to an increase in blood flow through the pulmonary valve. We report host cell infiltration and endothelialization of implanted decellularized pulmonary valves (dPV) and provide an experimental approach for the study of TEHVs using mouse models.
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James et al. (2015) studied Tissue-engineered heart valve evaluation (n=32). Loaded heterotopic heart transplant vs. Unloaded heterotopic heart transplant was evaluated on Pulmonary artery diameter (mm) (p=0.0367). The modified loaded heterotopic heart transplant model significantly increased pulmonary artery diameter (0.62 vs 0.44 mm, p=0.0367) and right ventricular stroke volume compared to the traditional unloaded model.
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