Key result
Addition of sucrose (413-825 mmol/L) to human serum allows plasma lipoproteins to be lyophilized without denaturation, creating a clear control serum suitable for clinical chemistry.
Adding sucrose to human serum before lyophilization preserves intact plasma lipoproteins, providing a stable control serum for lipid and apolipoprotein assays.
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May enable stable lyophilized controls for lipid assays; leaves open routine clinical chemistry adoption pending validation.
Addition of sucrose (413-825 mmol/L) to human serum allows plasma lipoproteins to be lyophilized without denaturation. A control serum so prepared is especially suited for use in monitoring determinations of apolipoproteins and quantitative lipoprotein electrophoresis. It is clear upon reconstitution; hence, the described procedure may also be useful for preparation of control sera for general clinical chemistry without de-lipoproteinization.
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Wieland et al. (1982) studied this question. Addition of sucrose to human serum was evaluated on Lyophilization without denaturation. Addition of sucrose (413-825 mmol/L) to human serum allows plasma lipoproteins to be lyophilized without denaturation, creating a clear control serum suitable for clinical chemistry.
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