Key result
Application of bradykinin to N1E-115 mouse neuroblastoma cells evoked sequential outward and inward membrane currents, identical to responses in NG108-15 hybrid cells.
Bradykinin induces sequential outward and inward membrane currents in N1E-115 mouse neuroblastoma cells, likely mediated by the dual effects of inositol trisphosphate and diacylglycerol.
Extends bradykinin current findings to N1E-115 cells; leaves open relevance to cardiovascular or neuronal physiology in vivo.
Application of bradykinin to voltage-clamped N1E-115 mouse neuroblastoma cells evoked sequential outward and inward membrane currents, accompanied by an increase and decrease of membrane conductance, respectively. Methacholine produced an inward current with a decreased conductance. The outward current response to bradykinin was imitated by intracellular inositol 1,4,5-trisphosphate (IP3). Bath application of phorbol dibutyrate induced an inward current and potentiated the response to IP3. We conclude that the response of these cells to bradykinin is identical to that of NG108-15 hybrid cells, and therefore may be attributed to the dual effects of inositol trisphosphate and diacylglycerol formed by hydrolysis of phosphatidylinositide.
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Higashida et al. (1987) studied this question. Bradykinin was evaluated on Membrane currents and conductance. Application of bradykinin to N1E-115 mouse neuroblastoma cells evoked sequential outward and inward membrane currents, identical to responses in NG108-15 hybrid cells.
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