Key result
Jugular vein tissue generated the highest number of myofibroblast cells and the largest amount of collagen per cell compared to tricuspid valve leaflet and carotid artery sources.
Jugular vein tissue is a promising and accessible source for generating myofibroblasts for tissue-engineered cardiac valves.
May support jugular vein as myofibroblast source for engineered valves; hypothesis-generating in animal models, needs functional validation.
The objective of this study was to evaluate the capacity of three clinically useful tissue sources: tricuspid valve leaflet (TVL), carotid artery (CA), and jugular vein (JV), to generate myofibroblasts for potential use in a tissue-engineered cardiac valve replacement. Tissue biopsies of clinically appropriate sizes obtained from juvenile sheep were used for this work. Cells obtained from all three tissue sources exhibited a myofibroblast phenotype in vitro, as demonstrated by their immunoreactivity with antibodies directed against vimentin, alpha-smooth muscle actin, fibronectin, and chondroitin sulfate. Protein synthesis characteristics were defined for the key extracellular matrix components: collagen, glycosaminoglycans, and elastin. Among the three sources, JV generated the highest numbers of cells, and JV cells produced the largest amount of collagen per cell. These data suggest that venous tissue, with its relative ease of accessibility, may generate myofibroblasts potentially useful for the interstitial cellular component of a tissue-engineered cardiac valve.
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Hoffman–Kim et al. (2005) studied Tissue-engineered cardiac valve replacement. Jugular vein (JV) myofibroblasts vs. Tricuspid valve leaflet (TVL) and carotid artery (CA) myofibroblasts was evaluated on Myofibroblast phenotype, cell yield, and collagen production. Jugular vein tissue generated the highest number of myofibroblast cells and the largest amount of collagen per cell compared to tricuspid valve leaflet and carotid artery sources.
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