Key result
OLF-1, OLF-2, and vanadium diascorbate produced a slow increase in intracellular free calcium in porcine IMCD cells, similar to the effects of ouabain.
Why the study?
Do urinary ouabain-like factors and vanadium diascorbate affect calcium mobilization in porcine inner medullary collecting duct cells compared to ouabain and vasopressin?
Population
Cultured porcine inner medullary collecting duct cells isolated by hypotonic treatment and density gradient…
Comparison
Urinary ouabain-like factors and vanadium… vs Ouabain and vasopressin
Design
Preclinical
Follow-up
4 minutes
Authors
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Hypothesis-generating for OLFs in renal calcium signaling; does not support clinical translation without human validation.
Do urinary ouabain-like factors and vanadium diascorbate affect calcium mobilization in porcine inner medullary collecting duct cells compared to ouabain and vasopressin?
Urinary ouabain-like factors and vanadium diascorbate produce a slow increase in intracellular calcium in IMCD cells, similar to ouabain, consistent with Na-K-ATPase inhibition.
Kramer et al. (1998) studied this question. Ouabain-like factors (OLF-1, OLF-2) and vanadium diascorbate vs. ouabain and vasopressin (AVP) was evaluated on free calcium concentration [Ca2+]i. OLF-1, OLF-2, and vanadium diascorbate produced a slow increase in intracellular free calcium in porcine IMCD cells, similar to the effects of ouabain.
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