Key result
Coupled translation of the M2-2 open reading frame occurs in APV and PVM at significantly lower efficiencies than in RSV due to sequence-dependent differences in the upstream M2-1 coding region.
Population
Chimeric M2-1 genes from respiratory syncytial virus, avian pneumovirus, and pneumonia virus of mice
Design
Preclinical
Authors
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No immediate clinical implications; leaves open whether M2-1 sequence differences modulate pneumovirus fitness or vaccine design across species.
Coupled translation of the M2 mRNA in pneumoviruses depends on specific upstream coding sequences and secondary structures.
Gould et al. (2007) studied this question. M2 mRNA chimeric constructs and mutations vs. Wild-type M2 mRNA constructs was evaluated on CAT reporter protein expression levels. Coupled translation of the M2-2 open reading frame occurs in APV and PVM at significantly lower efficiencies than in RSV due to sequence-dependent differences in the upstream M2-1 coding region.
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