What question did this study set out to answer?

Determine the enzymic pathways responsible for adenylate breakdown and characterize their regulation by cellular nucleotides in rat heart extracts.

November 1, 1967Journal of Biological ChemistryOpen Access

Adenylate Deaminase

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Authors

RBRichard M. BurgerRutgers, The State University of New Jersey JLJohn M. LowensteinBrandeis University

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Implication

Biochemical analysis reveals dual pathways of adenylate degradation regulated by ATP and GTP in rat heart tissue, indicating coordinated metabolic control of nucleotide turnover.

Key Points

Determine the enzymic pathways responsible for adenylate breakdown and characterize their regulation by cellular nucleotides in rat heart extracts.
Incubated rat heart tissue extracts with tritium- and phosphorus-32-labeled adenylate in the presence and absence of ATP, GTP, and adenosine.
Separated regulated adenylate deaminase from adenosine deaminase using gel filtration chromatography.
Assayed the parallel degradation pathways in lung tissue extracts.
Identified two degradation pathways in heart and lung extracts: one via inosinate and the other via adenosine, both yielding inosine.
ATP selectively activates adenylate deaminase and inhibits adenylate phosphatase, steering adenylate hydrolysis toward inosinate formation.
GTP reverses ATP-mediated activation of adenylate deaminase and inhibits adenylate phosphatase, while neither nucleotide affects adenosine deaminase.

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Burger et al. (1967) studied this question.

synapsesocial.com/papers/6a940847a0bd65b5cab93dc6 https://doi.org/10.1016/s0021-9258(18)99423-8

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Implication

Biochemical analysis reveals dual pathways of adenylate degradation regulated by ATP and GTP in rat heart tissue, indicating coordinated metabolic control of nucleotide turnover.

Adenylate Deaminase

Discussion

Member takes

Implication

Key Points

Cite This Study

Adenylate Deaminase

Discussion

Member takes

Implication

Key Points

Cite This Study

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