Key result
High-concentration D-glucose (20-30 mM) inhibited 60-70% of NO production in mouse glomerular endothelial cells within 15-30 minutes, an effect prevented by PKC-beta inhibition.
Population
Mouse kidney slices (150-200 microm)
Comparison
High-concentration (20-30 mM) D-glucose vs 5 mM D-glucose or 20-30 mM L-glucose
Design
Preclinical
Follow-up
15-30 min
Authors
Loading...
May contribute to early glomerular endothelial dysfunction in diabetes; leaves open whether PKC-beta inhibition alters nephropathy progression in vivo.
High glucose concentrations rapidly suppress eNOS in renal glomerular capillary endothelial cells via a PKC-dependent mechanism, providing insight into the pathogenesis of diabetic nephropathy.
Chu et al. (2004) studied Diabetic nephropathy. High-concentration D-glucose vs. 5 mM D-glucose or L-glucose was evaluated on NO production. High-concentration D-glucose (20-30 mM) inhibited 60-70% of NO production in mouse glomerular endothelial cells within 15-30 minutes, an effect prevented by PKC-beta inhibition.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: