Key result
Binding of the influenza virus polymerase to the 5' end of viral RNA stimulates capped mRNA binding, but subsequent endonuclease activity requires interaction with both the 5' and 3' ends.
Population
Influenza virus polymerase (in vitro assays)
Design
Preclinical
Authors
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May guide influenza antiviral development; leaves open translation from animal models to human disease.
The study demonstrates that regulation of endonuclease activity by the influenza virus polymerase depends on specific template RNA binding.
Cianci et al. (1995) studied Influenza virus (in vitro). Template RNA (vRNA and cRNA) vs. No template RNA was evaluated on Capped mRNA binding and endonuclease activity. Binding of the influenza virus polymerase to the 5' end of viral RNA stimulates capped mRNA binding, but subsequent endonuclease activity requires interaction with both the 5' and 3' ends.
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