Key result
Small interfering RNA targeted to the polymerase gene was investigated for the suppression of feline calicivirus replication.
siRNA targeted to the polymerase gene of FCV-B dose-dependently suppresses viral replication in vitro.
Offers preclinical support for polymerase-targeted siRNA in FCV; leaves open in vivo efficacy and clinical translation.
Feline calicivirus (FCV) is a pathogenic microorganism that causes upper respiratory diseases in cats. Recently, an FCV infection with a high mortality rate has been confirmed, and there is need to develop a treatment for cases of acute infection. We evaluated whether the replication of FCV could be prevented by RNA interference. For this study, we designed an siRNA targeted to the polymerase region of the strain FCV-B isolated from a cat that died after exhibiting neurological symptoms. Cells transfected with siR-pol dose-dependently suppressed the replication of FCV-B. siR-pol suppressed its replication by suppressing the target viral RNA.
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Taharaguchi et al. (2012) studied Feline Calicivirus infection. Small Interfering RNA Targeted to Polymerase Gene was evaluated. Small interfering RNA targeted to the polymerase gene was investigated for the suppression of feline calicivirus replication.
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